Protein Nanocapsules Produced by Nanoprecipitation of Designed Suckerin-Silk Fusion Proteins

Ricardo Ramos (1) , Kenrick Koh (2) , Bartosz Gabryelczyk (2) , Luxiao Chai (1) , Kanagavel Deepankumar (2) , Xibo Yan (1) , Francois Ganachaud (1) , Ali Miserez (2) , Julien Bernard (1)

1 IMP - Ingénierie des Matériaux Polymères
2 Nanyang Technological University [Singapour]

Herein, we report on the precise design of a modular fusion protein amenable to the construction of nanocapsules by nanoprecipitation. The central squid suckerin-derived peptide block provides structural stability, whereas both termini from silk fibroins make the modular protein highly soluble at physiological pH, a critical requirement for the nanoprecipitation process. With this molecular design, nanocapsules consisting of suckerin-silk fusion protein shells and hexadecane or miglyol cores with sizes in the range 190-250 nm are straightforwardly built.

Mots clés: Nanocarriers; Spider silk

Domaines: Chimie; Polymères

Ricardo Ramos, Kenrick Koh, Bartosz Gabryelczyk, Luxiao Chai, Kanagavel Deepankumar, et al.. Protein Nanocapsules Produced by Nanoprecipitation of Designed Suckerin-Silk Fusion Proteins. ACS Macro Letters, 2021, 10 (5), pp.628-634. ⟨10.1021/acsmacrolett.1c00171⟩. ⟨hal-03264411⟩